Summary
Blastn of http://eagle.fish.washington.edu/cnidarian/pearl_te.fa against the oyster genome (v9) with the following output:
http://eagle.fish.washington.edu/cnidarian/oyster_v9_blastn_pearl
#TESTING Posts
!date
Fri Feb 14 13:55:00 PST 2014
local image?
Need to make blast database from entire oyster genome.
!makeblastdb -in /Volumes/web/cnidarian/oyster.v9.fa -dbtype nucl -out /Volumes/Bay3/Software/ncbi-blast-2.2.27\+/db/oyster_v9
Building a new DB, current time: 06/11/2013 11:56:32 New DB name: /Volumes/Bay3/Software/ncbi-blast-2.2.27+/db/oyster_v9 New DB title: /Volumes/web/cnidarian/oyster.v9.fa Sequence type: Nucleotide Keep Linkouts: T Keep MBits: T Maximum file size: 1000000000B Adding sequences from FASTA; added 11969 sequences in 33.6918 seconds.
!blastn -query /Volumes/web/cnidarian/pearl_te.fa -db /Volumes/Bay3/Software/ncbi-blast-2.2.27\+/db/oyster_v9 -out /Volumes/web/cnidarian/oyster_v9_blastn_pearl -outfmt 6 -evalue 1E-5 -num_threads 2 -task blastn
!head /Volumes/web/cnidarian/oyster_v9_blastn_pearl
CVA scaffold1347 87.17 265 29 4 203 465 62899 63160 4e-83 311 CVA scaffold1347 79.39 131 23 2 335 465 120974 120848 3e-22 109 CVA scaffold1347 87.36 87 11 0 131 217 62983 63069 9e-22 107 CVA scaffold1347 71.05 152 13 3 320 463 290562 290690 7e-17 91.5 CVA scaffold1347 82.19 73 13 0 136 208 259668 259740 2e-11 73.4 CVA scaffold1347 82.19 73 13 0 292 364 259668 259740 2e-11 73.4 CVA scaffold1347 92.50 40 2 1 1 39 287927 287966 5e-06 55.4 CVA scaffold492 86.42 265 31 3 203 465 658118 658379 5e-82 307 CVA scaffold492 84.68 222 30 2 203 422 959949 959730 2e-62 242 CVA scaffold492 75.00 276 59 4 195 465 175106 175376 6e-43 178
!wc -l /Volumes/web/cnidarian/oyster_v9_blastn_pearl
8769 /Volumes/web/cnidarian/oyster_v9_blastn_pearl